Interaction of huanglongbing and foliar applications of copper on growth and nutrient acquisition of citrus sinensis cv. valencia

Abstract

The following study was conducted in 2016 and 2017 to determine the impact of frequent foliar copper (Cu) applications on Huanglongbing (HLB)-affected Citrus sinensis cv. Valencia orange. The experiment was conducted in a psyllid-free greenhouse with HLB-positive and non-HLB control trees grown in an Immokalee fine sand soil. The trees were well-maintained to promote health. Cu was applied to the foliage at 0x, 0.5x, 1x, and 2x the commercially recommended rates, which were 0, 46, 92, and 184 mM, respectively, with applications made 3x in both 2016 and 2017. The impact of HLB and Cu treatments on leaf and root Cu concentrations, vegetative growth, Candidatus Liberibacter asiaticus (CLasiaticus) genome copy number, and acquisition of other essential nutrients were determined. HLB caused the roots to acidify the soil more than non-HLB controls, which promoted Cu availability and promoted greater Cu concentrations in leaves and roots. HLB and Cu application treatments suppressed leaf area and total root length observable in rhizotron tubes such that, by the end of the experiment, leaf, stem, root, and whole-plant dry weights were reduced. HLB reduced foliar concentrations of calcium (Ca), magnesium (Mg), manganese (Mn), zinc (Zn) and possibly iron (Fe), but HLB did not affect root concentrations of these same essential nutrients. Cu application treatments did not affect leaf or root concentrations of other essential nutrients except foliar concentration of Fe, which may have been suppressed. Foliar applications of Cu are used to suppress Xanthomonas citri ssp. citri (Xcc) the causal agent of citrus canker, and the frequency of its use may need to be reconsidered in commercial groves.