Enhancing the yield of chlorophyll a from fresh spirulina using ultrasound-assisted extraction

Abstract

Spirulina is a spiral cyanobacteria with simple cell structure, making it easier to extract chlorophyll than other materials. Investigation the ultrasound assisted extraction of chlorophyll a was conducted on the following factors: type of solvent, concentration of solvent, ratio of solvent: material, time and temperature. Optimization by Design Expert the extraction of chlorophyll a from spirulina algae indicates the condition to obtain the highest amount of chlorophyll a of 15.07 mg/g was as followings: solvent ethanol concentration 99.7%, ratio solvent / material: 16/1, time 2.5 hours, temperature 52°C, ultrasonic frequency 40kHz.