Micropropagation of marsdenia brunoniana wight & Arn. - A rare antidiabetic plant

Abstract

Shoot multiplication of M. brunoniana Wight & Arn. was achieved from the nodal expiants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum expiant response and highest number of shoots per expiant was obtained on MS medium fortified with 1.0 mg/1 BAP. The highest degree of shoot proliferation was found to be 90%. The combination of BAP and Kn was also found to be effective for regeneration. The regenerated shoots were successfully rooted on MS supplemented with 0.5 mg/1 NAA, after sequential hardening; survival rate was 90%.