With a little help from a friend: Increasing HIV transduction of monocyte-derived dendritic cells with virion-like particles of SIVMAC

Abstract

Modification of dendritic cells (DCs) is a promising avenue for gene therapy purposes, given the versatility and the multiplicity of functions of these cells. In this study, we show that preincubation of monocyte-derived DCs with low amounts of non-infectious virion-like particles derived from the simian immunodeficiency virus (SIVMAC VLPs) increases up to 10-fold the efficiency of transduction by HIV-1 lentiviral vectors at low multiplicity of infections yielding up to 90% of transduced cells, in the absence of alterations of DCs behavior. This effect is restricted to DCs and specified by the viral accessory protein Vpx. Thus, preincubation with empty VLPs of SIVMAC can be used in transduction protocols to increase the efficacy of HIV-1-mediated modification of DCs. © 2006 Nature Publishing Group. All rights reserved.