Isolation and Characterization of Microbes with Biofertilizer Potential

Abstract

Microorganisms work incognito to maintain the ecological balance by active participation in carbon, nitrogen, sulphur and phosphorous cycles in nature. Sampleswere collected from nearriver Ganga mainly Hazipur, Sonepur and Vaishali. Sites were dry field, banana field, wetland and stagnant pond areas. Isolation was done by serial dilution method in nutrient agar plate. The isolates were found to be maximum in humus rich soil of S1 (Banana field) soil (Hazipur) and S2 (Sedimented pond) soil. A total of 111 microbial strains (bacteria) were isolated. Strain which showed the best result was tested for its' biofertilizer potential on the paddy (Oryza sativa, variety -Sugandha) individually through pot experiment. The test isolate namely, S1(C) was selected on the basis of their luxuriant growth on NA. This isolate showed a wide range of temperature for growth between 4 ْ C to 55 ْ C which suggests its potential as biofertilizer in field conditions where the organism has to survive under wide range of natural temperature fluctuations. The selected test isolate was Bacillus although molecular characterization (16s r-RNA studies) was done to confirm the genus and species of the selected isolate. On the basis of BLAST alignment of the sequenced nucleotides the isolate S1(C) was identified as Brevibacillusborstelensis. The reported literatures suggest that the B. borstelensisactsas PGPR.