Fatty Acid Composition of the Lipid Fractions from Bovine Serum Lipoproteins

Abstract

Weights and fatty acid composition of the lipid fractions have been determined from bovine serum lipoproteins separated ultracentrifugally at a solvent density of 1.063 g/ml and corresponding in eleetrophoretic mobility to α- and β-lipoproteins. The vast proportion of serum lipids was contained in the high-density lipoproteins (D > 1.063), with the marked exception of the triglycerides, which were evenly divided. Degree of saturation in the high-density lipoprotein total lipids was 27% and ranged from cholesteryl esters 8.4%, phospholipids 50%, nonesterified fatty acids 65%, to triglycerides 80%. The low-density lipoproteins (D < 1.063) showed a 74% degree of saturation in their total lipids which varied from phospholipids 73%, triglycerides 84%, nonesterified fatty acids 86% to cholesteryl esters 89%. Fatty acid distribution indicated a large concentration of linoleate in the total lipids and cholesteryl esters of the high-density lipoproteins. This concentration was dispersed in their other lipid fractions. The low-density lipoproteins contained chiefly palmitate and stearate in all lipid fractions. Triglycerides in both high- and low-density lipoproteins were notably similar in weight, saturation, and fatty acid composition. © 1961, American Dairy Science Association. All rights reserved.