Partial characterization of chitosanase from digestive tract of Channa striata

Abstract

The purpose of this study was to partially characterize chitosanase isolated from snakehead fish's (Channa striata) digestive tract. Two types of snakehead fish, domesticated and non-domesticated, were used as samples in order to assess the effect of feeding types on chitosanase activity. Chitosanase isolated from the stomach and intestine of domesticated snakehead fish had higher activity than non-domesticated samples with the value of 0.0395 U/mL and 0.0296 U/mL, respectively. The optimum chitosanase activity from the stomach and the intestine of domesticated fish was at pH 7. Lower optimum pH (6) was noticed for non domesticated samples. Chitosanase from both samples showed optimum activity at 70°C.