Uncovering potential differentially expressed miRNAs and targeted mRNAs in myocardial infarction based on integrating analysis

Abstract

Myocardial infarction (MI) is one of the leading causes of death globally. The aim of the present study was to find valuable microRNA s (miRNA s/miRs) and target mRNA s in order to contribute to our understanding of the pathology of MI. miRNA and mRNA data were downloaded for differential expression analysis. Then, a regulatory network between miRNA s and mRNA s was established, followed by function annotation of target mRNA s. Thirdly, prognosis and diagnostic analysis of differentially methylated target mRNA s were performed. Finally, an in vitro experiment was used to validate the expression of selected miRNA s and target mRNA s. A total of 19 differentially expressed miRNA s and 1,007 differentially expressed mRNAs were identified. Several regulatory interaction pairs between miRNA and mRNA s were identified, such as hsa-miR- 142-2p-long-chain-fattyacid-C oA ligase 1 (AC SL1), hsa-miR- 15a-3p-nicotinamide phosphoribosyltransferase (NA MPT), hsa-miR- 33b-5p-regulator of G-protein signaling 2 (RGS2), hsa-miR- 17-3p-Jun dimerization protein 2 (JDP2), hsa-miR- 24-1-5p-aquaporin-9 (AQP9) and hsa-miR-34a-5p-STAT1/AKT3. Of note, it was demonstrated that AC SL1, NA MPT, RGS2, JDP2, AQP9, STAT1 and AKT3 had diagnostic and prognostic values for patients with MI. In addition, STAT1 was involved in the 'chemokine signaling pathway' and 'Jak-STAT signaling pathway'. AKT3 was involved in both the 'MAPK signaling pathway' and 'T cell receptor signaling pathway'. Reverse transcription-quantitative PCR validation of hsa-miR- 142-3p, hsa-miR- 15a-3p, hsa-miR- 33b-5p, AC SL1, NA MPT, RGS2 and JDP2 expression was consistent with the bioinformatics analysis. In conclusion, the identified miRNAs and mRNAs may be involved in the pathology of MI.