Actividad antioxidante de hidrolizados enzimáticos de plasma bovino obtenidos por efecto de alcalasa® 2.4 L

Abstract

The antioxidant activity of bovine plasma hydrolysates (BPH) obtained with Alcalase 2.4 L at different hydrolysis degrees was evaluated. The hydrolysates were fractioned through ultrafiltration membranes and were purified by ion-exchange chromatography, with subsequent reverse-phase high performance liquid chromatography. The results showed that the hydrolysates of bovine plasma obtained under the established hydrolysis conditions, had strong scavenging ability on 2,2-azino bis-(3-ethylbenzothiazoline)-6-sulfonic acid free radicals and a great reduction power compared with proteins of non-hydrolyzed plasma. Additionally, it was found that the antioxidant activity increases as function of the hydrolysis degree, and that this activity remains after in-vitro digestion. The processes described allow obtaining a peptid with antioxidant activities similar to that of conventional commercial antioxidants.