Método para preservação da viabilidade e atividade antagônica de trichoderma stromaticum, agente de biocontrole da vassoura-de-bruxa do cacaueiro

Abstract

After four years of preservation by using dried cocoa brooms in fridge at 5°C, the viability of four isolates (Ts1606, Ts3107, Ts0108, Ts2705) of Trichoderma stromaticum was evaluated based on germination of conidia, mycelium growth in a culture medium and antagonistic activity on dry cocoa brooms. All the isolates preserved maintained viability, showing normal growth, sporulation and antagonistic activity against Crinipellis perniciosa.. These results indicate the efficience of the method for the long term preservation of T. stromaticum, maintaining their morphological, physiological, and antagonistic characteristics.