Comparison of diagnostic tests for the detection of equine infectious anemia antibody.

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Abstract

Two diagnostic tests are approved for detecting antibody to equine infectious anemia virus: the agar-gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (ELISA). A total of 420 sera from National Veterinary Services Laboratories check sets were tested with the AGID and competitive ELISA. A 100% correlation was obtained. The AGID and competitive ELISA were further used to test difficult samples with low levels of equine infectious anemia antibody (weak positives). A third test (Western blot) was also used with these weak positive samples to resolve any discordant results.

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Matsushita, T., Hesterberg, L. K., Porter, J. P., Smith, B. J., & Newman, L. E. (1989). Comparison of diagnostic tests for the detection of equine infectious anemia antibody. Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 1(1), 50–52. https://doi.org/10.1177/104063878900100114

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