Preparative isolation and gas chromatography-mass spectrometry analysis of triterpenoids in kansui radix

Abstract

Two triterpenoids, euphol and tirucallol were isolated from Kansui Radix (Euphorbia kansui Liou, Euphorbiaceae) by a modified isolation method. These constituents were used as marker substances for qualffy control of Kansui Radix using a home-developed gas chromatography-mass spectrometry (GC-MS) method. The linear calibranon ranges were 50.0-400.0 μg/mL (r=0.9988) for euphol and 20.0-200.0 μg/mL (r=0.9976) for tirucallol. The relative standard deviations of two marker substances for intraday and interday analyses were 0.90-3.07% and 3.93-8.17%, respectively. Recoveries were euphol 98,3±1.2%, and tirucallol 84.8±.0%. The contents of two marker substances in seven crude drugs of Kansui Radix were euphol 0.10-0.19%, and drucallol 0.05-0.07%.