Identification and determination of phenolic acids content in mango "golek" leaves ethanol extract

Abstract

Ethanol extract from mango "golek" leaves (Mangifera indica L) was obtained by maceration in ethanol 96%. Phenolic acid was isolated from the extract by hydrolysis with and without a hydrolyzing agent (TH). Two different hydrolyzing agents were used separately, i.e., sulfuric acid (HA) and sodium hydroxide (HB). Identification of phenolic acid was made with TLC and High-performance liquid chromatography (HPLC). Determination of phenolic acid content was carried out using TLC Scanner. Standard phenolic acid was used as a control. Phenolic acid compounds were obtained in the HA, HB and TH isolate namely gallic acid. The total gallic acid content was 35.88% consisting of HA, HB and TH fraction of 3.46%; 12.42%; 19.98%.