Redox behavior of anticancer chalcone on a glassy carbon electrode and evaluation of its interaction parameters with DNA

Abstract

The interaction of anticancer chalcone [AMC, 1-(4′-aminophenyl)-3-(4- N,N-dimethylphenyl)-2-propen-1-one] with DNA has been explored using electrochemical, spectroscopic and viscometric techniques. A shift in peak potential and decrease in peak current were observed in cyclic voltammetry and hypochromism accompanied with bathochromic shift were noticed in UV-Vis absorption spectroscopy. These findings were taken as evidence for AMC -DNA intercalation. A binding constant (K) with a value of 6.15 × 10 5 M -1 was obtained from CV data, which was also confirmed by UV-Vis absorption titration. Moreover, the diffusion coefficient of the drug with and without DNA (D b and D u), heterogeneous electron transfer rate constant (k o) and electron affinity (A) were also calculated from electrochemical data. © 2008 by the authors; licensee Molecular Diversity Preservation International.