Application of an optimized and highly discriminatory method based on arbitrarily primed PCR for epidemiologic analysis of methicillin-resistant Staphylococcus aureus nosocomial infections

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Abstract

The optimization of an arbitrarily primed PCR method for typing 96 methicillin-resistant Staphylococcus aureus (MRSA) isolates was compared with pulsed-field gel electrophoresis. Identical results in the differentiation of MRSA clones and identification of the main cluster that included 82 strains (88% of patients) were obtained by both techniques.

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Olmos, A., Camarena, J. J., Nogueira, J. M., Navarro, J. C., Risen, J., & Sanchez, R. (1998). Application of an optimized and highly discriminatory method based on arbitrarily primed PCR for epidemiologic analysis of methicillin-resistant Staphylococcus aureus nosocomial infections. Journal of Clinical Microbiology, 36(4), 1128–1134. https://doi.org/10.1128/jcm.36.4.1128-1134.1998

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