Vitamin D by LC MS MS

Abstract

> The sunshine vitamin has been hogging the limelight; > > As not only a nutrient, but a pro-hormone in its own right. > > The major circulating form in the plasma 25-hydroxy vitamin D, > > Is the sum of two species derived from vitamins D2 and D3. > It is this hydroxyl form that reflects its nutritional state; > > As we sink into a deficiency its levels decline and abate. > > Its levels help to diagnose, a sub-clinical deficiency disease, > > And to discriminate, a sufficiency state, from toxicity, with ease. > With enormous advances made in the technique of liquid chromatography; > > Coupled with detection and quantification using mass spectrometry; > > Robust methods have been developed to quantify 25-hydroxy D, > > Bringing …