The Formulation and Evaluation of High-Fat Pellet on Lipid Profiles and Body Mass Index of Male Wistar Rats

Abstract

This study aims to explore the manufacturing of high-fat pellets for obesity induction diets in male Wistar rats and determine their effects on lipid profiles and body mass index. It was conducted using an experimental laboratory method with posttest with control group. The formulation and evaluation of the physico-chemical characteristics of the high-fat pellets (HFD) were conducted in September 2019. In this study, the 28 male Wistar white rats used were 2 months old with 150-200 g bodyweight. The rats were acclimatized for 7 days and divided into 2 groups, namely the P0 group which was fed with standard PARS CP594 confeed as many as 7 rats (P0) and the P1 group which was fed high-fat diet (HFD FII) as many as 21 rats, each at 30 g/head/day for 8 weeks.. The result showed that the mean fat content of Formula II pellets (HFD FII), 25.44% ± 0.16 was higher than Formula I pellet (HFD FI) (22.55% ± 0.16) and 3% standard feed. Furthermore, the mean of body weight and BMI of obesity induction rat groups (P1) were significantly higher than the standard rat group (P0) (p <0.05). The feed consumption in the rat fed with HFD FII pellets was also higher than the standard group (P0), indicating that rats preferred the HFD FII pellets. The lipid profile of the obesity induction group showed higher total cholesterol, triglycerides, and LDL, while the HDL levels were significantly lower compared to the standard feed group (P0). Therefore, giving HFD FII pellets, which are a source of fat from butter, full cream milk powder, and eggs of purebred chickens for 8 weeks can make male Wistar rats obese and dyslipidemic.