An alternative method for determining the radiochemical purity of 99mTc-tetrofosmin

Abstract

The instant thin-layer chromatographic method of determining the radiochemical purity of 99mTc-tetrofosmin recommended by the manufacturer uses a mixture of acetone and dichloromethane (35:65 v/v) as the solvent and a silica gel (SG) strip as the solid phase. Currently, SG strips are not available commercially and an alternative solid phase is needed. The present study reports that silicic acid strips are an appropriate substitute for SG strips for the chromatographic method using the same solvent mixture but mixed in a reverse ratio (65:35 v/v). The 2 methods yielded similar values (within 1 SD) of radiochemical purity for 99mTc-tetrofosmin. © 2011 by the Society of Nuclear Medicine, Inc.