Molecular Reidentification of Endophytic Fungus LBKURCC40 Isolated from Dahlia's Tuber (Dahlia Variabilis) Using the Sequence of ITS rDNA

Abstract

Endophytic fungus LBKURCC40 isolated from dahlia tubers (Dahlia variabilis) was morphologically and molecularly identified. Molecular identification was carried out using ITS4 and ITS5 primers, furthermore, the homology level of LBKURCC40 isolates was checked using BLAST program and the suitability of BLAST results was tested with the TrichOKEY program. The results of showed that LBKURCC40 isolates were identified as Trichoderma asperellum with a homology level of 99%. Phylogenetic analysis was carried out with N-J tree bootstrap test with 10,000 repetitions, and LBKURCC40 isolate was obtained to have the closest kinship to Trichoderma asperellum strain Tr266B.