Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA

Abstract

The ability of Acinetobacter sp. strain BD413(pFG4ΔnptII) to take up and integrate transgenic plant DNA based on homologous recombination was studied under optimized laboratory conditions. Restoration of nptII, resulting in kanamycin-resistant transformants, was observed with plasmid DNA, plant DNA, and homogenates carrying the gene nptll. Molecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional DNA.