Distribution of hydrogenase genes in Desulfovibrio spp. and their use in identification of species from the oil field environment

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Abstract

The distribution of genes for [Fe], [NiFe], and [NiFeSe] hydrogenases was determined for 22 Desulfovibrio species. The genes for [NiFe] hydrogenase were present in all species, whereas those for the [Fe] and [NiFeSe] hydrogenases had a more limited distribution. Sulfate-reducing bacteria from 16S rRNA groups other than the genus Desulfovibrio (R. Devereux, M. Delaney, F. Widdel, and D.A. Stahl, J. Bacteriol. 171:6689-6695, 1989) did not react with the [NiFe] hydrogenase gene probe, which could be used to identify different Desulfovibrio species in oil field samples following growth on lactate-sulfate medium.

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Voordouw, G., Niviere, V., Ferris, F. G., Fedorak, P. M., & Westlake, D. W. S. (1990). Distribution of hydrogenase genes in Desulfovibrio spp. and their use in identification of species from the oil field environment. Applied and Environmental Microbiology, 56(12), 3748–3754. https://doi.org/10.1128/aem.56.12.3748-3754.1990

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