The Immunostimulatory Effects of Peronema canescens. Jack Leaves Extract in Mus musculus L. Using the Carbon Clearance Method

Abstract

Sungkai leaf extract (Peronema canescens Jack.) has been used traditionally to cure various diseases. This research aimed to determine the immunostimulant effect of sungkai leaf extract using the carbon clearance method, the total leukocyte cell count, and the percentage of leukocyte cell types. Twenty-five male mice divided into 5 groups were used for this study. The extract was administered orally for 6 days at 25, 50, and 100 mg/kgbw, Na CMC 0.5% as negative control, and Stimuno 50 mg/kgbw as a comparison (positive control) group. The phagocytic index, total leukocytes, and the percentage of leukocyte cell types were determined on the 7th day. The phagocytotic index value was analysed by one-way ANOVA followed by Duncan’s post-hoc test. The increase in phagocytosis index showed that the effect of each dose was significantly different (p< 0.05) compared to the control group. The highest phagocytosis index was obtained from a 100 mg/kg bw dose. There was a significant difference (p<0.05) in the total leukocyte cell count and the percentage of leukocyte cell type. Sungkai leaf extract (Peronema canescens Jack.) had an immunostimulant effect on male white mice (Mus musculus L.).