Fatty Acid Bioconversion and Scaling-Up Effects of Swine Manure Treatment with Black Soldier Fly Larvae

Abstract

Black soldier fly larvae (BSFL) treatment offers a promising avenue for manure valorization. However, there is a lack of larval density studies and ton-scale exploration in swine manure bioconversion. This study delves into the efficiency of larval fatty acid (FA) bioconversion, examining the impact of larval density on a kilogram scale and extending the analysis to a ton scale. Across a range of 50 to 600 larvae/kg, the larval FA content decreased from 15.3% to 7.85%. The peak larval FA yield, at 3.04% (based on manure dry matter), occurred at a density of 200 larvae/kg. Both low (50 larvae/kg) and high (600 larvae/kg) densities adversely affected BSFL bioconversion performance. Dominant larval FAs included C12:0 (39.7%), C16:1 (24.2%), C18:1 (17.5%), and C16:0 (8.3%). The scaling-up process maintained a consistent larval FA content and composition but resulted in decreased larvae FA yield due to increased larval mortality. Ultimately, each ton of swine manure yielded 12.4 kg of fresh larvae or 0.71 kg of larval FA components, corresponding to a 1.14% larval FA yield. This study underscores the feasibility of upscaling swine manure treatment using BSFL for FA bioconversion and emphasizes the necessity for large-scale studies to enhance larval survivorship and bioconversion efficiency.