Detection methods for mycotoxins in cereal grains and cereal products

  • Pascale M
N/ACitations
Citations of this article
178Readers
Mendeley users who have this article in their library.

Abstract

Analytical methods for mycotoxins in cereals and cereal-based products require three major steps, including extraction, clean-up (to eliminate interferences from the extract and concentrate the analyte), and detection/determination of the toxin (by using suitable analytical instruments/technologies). Clean-up is essential for the analysis of mycotoxins at trace levels, and involves the use of solid phase extraction and multifunctional (e.g. MycoSep?) or immunoaffinity columns. Different chromatographic methods are commonly used for quantitative determination of mycotoxins, including gas-chromatography (GC) coupled with electron capture, flame ionization or mass spectrometry (MS) detectors (mainly for type-A trichothecenes), and high-performance liquid chromatography (HPLC) coupled with ultraviolet, diode array, fluorescence or MS detectors. The choice of method depends on the matrix and the mycotoxin to be analyzed. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is spreading rapidly as a promising technique for simultaneous screening, identification and quantitative determination of a large number of mycotoxins. In addition, commercial immunometric assays, such as enzyme-linked immunosorbent assays (ELISA), are frequently used for screening purposes as well. Recently, a variety of emerging methods have been proposed for the analysis of mycotoxins in cereals based on novel technologies, including immunochromatography (i.e. lateral flow devices), fluorescence polarization immunoassays (FPIA), infrared spectroscopy (FT-NIR), molecularly imprinted polymers (MIPs) and optical biosensors.Metode analize mikotoksina u zitaricama i proizvodima od zita se sastoje iz tri postupka: ekstrakcije, preciscavanja (kako bi se uklonile interferencije ekstrakta i koncentrisao analit) i detekcije/odredjivanja toksina (koriscenjem odgovarajucih analitickih instrumenata/tehnologije za analizu). Preciscavanje je kljucni postupak u analizi mikotoksina pri njegovim koncentracijama u tragovima koji obuhvata primenu ekstrakcije cvrstom fazom i multifunkcionalne (npr. MycoSep?) ili imunoafinitetne kolone. Razlicite hromatografske metode se koriste za kvantitativno odredjivanje mikotoksina, ukljucujuci gasnu hromatografiju (GC) uz koriscenje detektora sa zahvatom elektrona, plameno jonizacione detektore ili masenu spektrometriju (uglavnom za trihotecene tipa A) kao i tecnu hromatografiju visokog ucinka (HPLC) uz koriscenje UV, DAJD, fluorescentnih i MS detektora. Izbor metode zavisi od matriksa i mikotoksina koji se analizira. Tecna hromatografija u paru sa masenom spektrometrijom (LC-MS/MS) je tehnika koja najvise obecava u slucaju simultanog skeniranja, identifikacije i kvantitativnog odredjivanja velikog broja mikotoksina. Osim toga, komercijalna imunometrijska ispitivanja, kao sto je enzimski imunosorpcioni test (ELISA), takodje se cesto koriste u svrhe skeniranja. U poslednje vreme velik broj metoda koje se baziraju na novim tehnologijama mogu se koristiti za analizu mikotoksina, a medju njima su imunohromatografija (npr. lateralni protocni uredjaji), imuno testovi sa polarizacijom fluorescentne emisije (FPIA), infracrvena spektroskopija (FT-NIR), polimeri sa molekularnim otiskom (MIPs) i opticki biosenzori.

Cite

CITATION STYLE

APA

Pascale, M. (2009). Detection methods for mycotoxins in cereal grains and cereal products. Zbornik Matice Srpske Za Prirodne Nauke, (117), 15–25. https://doi.org/10.2298/zmspn0917015p

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free