Targeted resequencing for large-scale genomic studies: accelerating NGS throughput using molecular barcodes

Abstract

Next-generation sequencing (NGS) is a powerful tool for the massively parallel investigation of specific genomic loci. However, sequence capture methodologies are required for time- and cost-effective sequence analysis in high-throughput genomic projects. HybSelect is a microarray-based sequence capture strategy that enables selective enrichment of a desired target region with increased sample numbers. Adding sample barcoding technology to the automated HybSelect approach further increases sample throughput and efficiently amends NGS technologies involved in large-scale discovery studies.