Novel approach for investigation of antibiotic residue in broilers grown under different agro-ecological conditions

Abstract

The purpose of this research work was to detect antibiotic remnants from chicken meat and to quantify them. For this purpose, the chicken was collected from three poultry farms in Lahore Sheikhupura area. These chickens were categorized according to quality (A, B and C grade) and on the basis of the day of collection (7th, 21st and 35th Growth day). The qualitative analysis was done by agar well diffusion method against B. subtilis, P. aeruginosa, E. coli and S. aureus. Analysis was done for muscles, kidney and liver samples. The highest residual potential was observed for liver extract (7th day) while the minimum for muscle extract (35th day). The samples with the highest zones of inhibition against pathogens were analyzed by HPLC.