Evaluation of the role of mirna-21 levels as a potential diagnostic biomarker for colorectal cancer associated with prognosis

Abstract

Colorectal cancer one of the most common and aggressive tumor diagnosed in humans. The potential role has been suggested that circulating microRNAs assuring diagnostic markers for early detection of colorectal cancer.Hereabouts, this investigation intends to study the performance of miRNA-21 in CRC. Fifty blood samples were collected from individuals with colon cancer who enrolled in the Teaching Laboratories of the Medical City/Baghdad from June 2018 to September 2019. Taq Man microRNA Real-time PCR was utilized to recognize the expression of miRNA-21 in the patient’s plasma. Representative in the expression of miRNA-21 by gender and pathological grading were not statistically important (P>0.05). Moreover, alterations in the expression of miRNA-21 amongst patients and control were significances statistically (P<0.05). While a higher risk of the disease was associated with high miRNA-21 and correlated with poor prognosis. The AUC for miRNA-21 was 0.657. The optimal cut-off value was 1.961-fold with sensitivity and specificity 64%, 68% respectively. In Conclusion, the high miRNA-21 expression might be a prognostic marker of colon cancer patient consequently; high expression of miRNA-21 was associated with poor prognosis of patients with colorectal cancer.