The effect of respiratory gases and incubation temperature on early stage embryonic development in sea turtles

Abstract

Sea turtle embryos at high-density nesting beaches experience relative high rates of early stage embryo death. One hypothesis to explain this high mortality rate is that there is an increased probability that newly constructed nests are located close to maturing clutches whose metabolising embryos cause low oxygen levels, high carbon dioxide levels, and high temperatures. Although these altered environmental conditions are well tolerated by mature embryos, early stage embryos, i.e. embryos in eggs that have only been incubating for less than a week, may not be as tolerant leading to an increase in their mortality. To test this hypothesis, we incubated newly laid sea turtle eggs over a range of temperatures in different combinations of oxygen and carbon dioxide concentrations and assessed embryo development and death rates. We found that gas mixtures of decreased oxygen and increased carbon dioxide, similar to those found in natural sea turtle nests containing mature embryos, slowed embryonic development but did not influence the mortality rate of early stage embryos. We found incubation temperature had no effect on early embryo mortality but growth rate at 27°C and 34°C was slower than at 30°C and 33°C. Our findings indicate that low oxygen and high carbon dioxide partial pressures are not the cause of the high early stage embryo mortality observed at high-density sea turtle nesting beaches, but there is evidence suggesting high incubation temperatures, particularly above 34°C are harmful. Any management strategies that can increase the spacing between nests or other strategies such as shading or irrigation that reduce sand temperature are likely to increase hatching success at high-density nesting beaches.