Association study of polymorphisms in ABCA7, clusterin, and MS4A6A genes with Alzheimer's disease in the Egyptian population

Abstract

Alzheimer's disease is a genetically heterogeneous brain disease which is associated with a decrease in thinking capacity and changes in personality. Various gene polymorphisms could be associated with the occurrence of Alzheimer's disease. Objectives: This study aimed at exploring the association of ABCA7 rs3764650, CLU rs11136000, and MS4A6A rs610932 genetic variants with AD in a sample of the Egyptian population. Methods: DNA extraction was done from peripheral blood. Genotyping was done using PCR followed by restriction fragment length polymorphism (RFLP) in 100 Alzheimer patients of age 60 years old or above and 100 cognitively normal controls. DNA sequencing was done for eight samples with different genotypes to confirm the results. Results: No significant difference was found in genotypic or allelic distribution of CLU rs11136000 between patients and controls. the frequency of CLU CC genotype among AD patients, was lower in Apo E 4 carriers compared to Apo E 4 non-carriers with a significant difference (p=0.003). No significant difference was found in genotypic or allelic distribution of ABCA7 rs3764650 and MS4A6A rs610932 between patients and controls. Conclusion: We concluded that AD not associated with Clustrin rs11136000, ABCA7 rs3764650 and MS4A6A rs610932 genes polymorphism.