Administration of ethanol extract of mustard greens (Brassica rapa L) leaves increased Superoxide Dismutase levels in Hyperglycemic rat

Abstract

Mustard green leaves (Brassica rapa L) is one of the Indonesian medicinal plants that can be used as an antidiabetic drug. This study aims to prove that administration of ethylacetate fracion of ethanol extract of mustard greens (Brassica rapa L) leaves increased superoxide dismutase (SOD)'level in hyperglycemic Wistar rats. Mustard green leaves powder (Brassica rapa L) was extracted by maceration using 96% ethanol to yield crude ethanol extract. 30 Wistar rats were divided into 5 groups consisting of 6 rats each group. Negative group, P0 (received food standart only), positive control, P1 (induced with streptozotocin and given glibenclamide drug); P2, P3, and P4 were treatment groups (induced with streptozotocin and given ethanol extract of mustard green at doses of 0.5, 2.0, and 5.0 mg/KgBw/day respectively). The dose of streptozotocin used to induce hyperglycemia in all rats was 125 mg/KgBw/day. The level of superoxide dismutase was examined before inducing streptozotocin and after the rats hyperglycemic. The ethanol extract was then fractionated into ethyl acetate fraction and then idenfified using GC-MS. The result showed that administration of ethanol extract at doses 5 mg/KgBW significannly increased SOD level(4.13 ± 1.18 ng/mL) of hyperglycemic rats as compared to negative control (2.75 ± 0.55ng/mL). Analysis GC-MS spectra of the ethylacetate fraction of ethanol extract of mustard green showed 6 major peaks assigned as vinyl propionste, buthyl formste, 2-methoxy4-vinylphrnol, 13-oxa-dispiro{5.0.5.1}trican-1-one, 1-methyl isoeugenol and 3-isopropoxy-5-methyl-phenol.