Isolation of nuclei for chromatin analysis in fission yeast

Abstract

The methods available for analysis of the chromatin of Schizosaccharomyces pombe are time consuming (> 8 h) and/or result in some degradation of the chromatin. Here we report an optimised method for the preparation of spheroplasts and the isolation of nuclei which takes < 25 min and is suitable for analysis of chromatin structure by micrococcal nuclease, restriction endonuclease or by immunoprecipitation.