Diversity and enterotoxigenicity of Staphylococcus spp. associated with Domiati cheese

Abstract

A total of 87 samples of fresh and stored Domiati cheese (an Egyptian soft cheese) were examined for the presence of Staphylococcus spp. Fifteen Staphylococcus isolates identified as S. aureus (2 isolates). S. xylasus (4), S. caprae (4), and S. chromogenes (5) were recovered from 15 cheese samples. The S. aureus isolates were resistant to penicillin G and ampicillin, and one isolate was also resistant to tetracycline. S. aureus isolates harbored classical staphyhlococcal enterotoxin (SE) genes (sea and seb) and recently characterized SE-like genes (selg, seli, selm, and selo). One S. aureus isolate contained a single SE gene (sea), whereas another isolate contained five SE genes (seb, selg. seli, selin, and selo). These results suggest that Domiati cheese is a source for various Staphylococcus species. including S. aureus strains that could be enterotoxigenic. Copyright ©, International Association for Food Protection.