Effect of sucrose in strawberry micro-propagation using platform bioreactor under temporary immersion system

Abstract

The study was carried out in the tissue culture laboratories at the College of Agriculture / University of Kufa during the period from April 2020 to October 2021 where the aim was to assess the efficiency of micropropagation of strawberries by adding different concentrations of sucrose (30, 40 and 50 g.L-1) to the nutrient medium in combination with different cultivation systems: Semi-solid media system and temporary immersion system using the Plantform bioreactor. The results showed that both the highest concentration of sucrose and the culture system under the medium immersion, individually or combined, were the highest and significantly effective in increasing the rate of number of shoots (runners), leaf content of macro and minor elements, total soluble sugars, plant pigments and fresh weight of biomass. Generally, the highest values were recorded in the shoots grown in liquid media supplied with 50 g.L-1 sucrose under temporary immersion in the PlantForm bioreactor compared with those grown in semi-solid media.