Phytochemical Characterization of Hydroponic Naked Barley, Analysis of Antioxidant Potential

Abstract

Background: In the present quantitative and qualitative study, to compare the new variety of naked barley with other cereals (durum wheat, commun wheat, coated barley). Regarding the richness in phytonutrients including mainly flavonoids, polyphenols, tannins condensed in a hydroponic culture. Methods: The experiment was conducted in Laboratory of Life Sciences and Techniques, Souk Ahras University in Algeria during 2019–2022. The antioxidant power was tested according to the DPPH free radical scavenging method. The results obtained from the quantitative study of methanolic extracts show that varieties rich in tannins and flavonoids have a significant antioxidant activity than a variety rich only in total polyphenols. Qualitative studies based on HPLC of these compounds allow us to visualize phenolic traces of our extracts, this analysis showed that our extracts mainly contain flavonoids of the flavone and flavonol type, potentially interesting for their biological properties. Result: The results reported the existence of a very highly significant difference (P<0.001) between IC50 of the new variety Chaiir Ennabi (Naked Barley) compared to ascorbic acid, which shows a lower antioxidant activity of this variety. Barley variety Barbarous (coated barley) had the highest antioxidant activities and the highest contents of phenolic compounds.