Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures

Abstract

After mutagenesis, surviving yeast cells are grown on plates at 25°C and later exposed to 37°C. The plates are then overlaid with a soft agar containing p nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies.