Seed protein variability in safflower

Abstract

Total seed proteins in two safflower species (Carthamus tinctorius L. and C. lanatus L) have been separated by the SDS-PAGE method. Their molecular masses ranged from 120 to 20 kDa. All C. tinctorius genotypes under study exhibited identical electrophoretic patterns which differed from the pattern exhibited by the wild species C. lanatus in the number and position of protein bands. Differences in protein profiles occurred in regions around 60 kDa, from 43 to 36 kDa and around 30 kDa. Statistically significant differences in seed protein content were found among safflower genotypes from different countries as well as among genotypes from the same country but from different sites. The highest seed protein content was found in a genotype originating from the USA.Ukupni proteini semena dve ispitivane vrste safrana (Carthamus tinctorius L. i C. lanatus L) su razdvojeni metodom SDS-PAGE i njihova molekulska masa se kretala od 120 do 20 kDa. Svi gajeni genotipovi vrste C. tinctorius imali su identicne elektroforetske putanje koje su se razlikovale po broju i polozaju proteinskih traka od divlje vrste C. lanatus. Razlike u proteinskim profilima javljale su se u regionima: oko 60 kDa, od 43-36 kDa i 30 kDa Utvrdjena je statisticki signifikantna razlika u sadrzaju proteina u semenu safrana poreklom iz razlicitih zemalja, kao i izmedju semena poreklom iz iste zemlje sa razlicitih lokaliteta. Najveci sadrzaj proteina utvrdjen je u semenu poreklom iz SAD.