A method for aseptic culture of bud explants pogestemon cablin benth Var Tapak Tuan, Aceh, Indonesia

Abstract

Pogostemon cablin Benth var Tapak Tuan, Aceh, Indonesia is a plant herb potentially to be developing with culture method. However, culture of this cultivar (stem and leave of bud0 explants sampled from outdoor trees is sometimes challenges due to contamination not overcome by standard surface-sterilization. An Improve method, involving a 5-minute immersion of explants in 5% (v/v) detergent, 1% fungicide and bactericide, 5% NaClO and 0.5% antibioticswhich does not interfere with normal explant and callus development, is described in detail.