The anti-proliferative activity of vitis vinifera leaves of methanol extract alone and in combination with doxorubicin against liver cancer cell line

Abstract

The aim of this study was to investigate the capability of Vitis vinifera leaves of methanol, water extracts alone and in combination with doxorubicin to inhibit HepG2 liver cancer cell line proliferation, and to identify the possible mechanism of action. The antiproliferative activity of the methanol and water extracts tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay on liver cancer cell line (HepG2). Dose response relationship was shown. Significant cytotoxic activity of methanol and water extracts has identified on HepG2, the IC50 were 7.80μg/ml and 20.8μg/ml respectively. When in combination with doxorubicin Dose response relationship was shown. Significant cytotoxic activity of the methanol extract combination but no significant cytotoxic activity of the water extracts combination, the IC50 were 4.2μg/ml and 31.5μg/ml respectively. These finding showed that methanol and water extracts alone had a significant dose-dependent efficiency against the growth of the cells HepG2; At the same time in combination with doxorubicin methanol extract significant dose-dependent efficiency against the growth of the cells HepG2 (synergistic). However, the water extracts combination did not show any cytotoxic action at the applied dose, so no toxic effect against the HepG2 cell line can be expected in vitro (contrast action).