Acidipila rosea gen. nov., sp. nov., an acidophilic chemoorganotrophic bacterium belonging to the phylum Acidobacteria

Abstract

Two strains of aerobic acidophilic chemoorganotrophic bacteria designed strains AP8T and AP9 were isolated from acid mine drainage and acidic soil, respectively. These isolates were Gram-negative, nonmotile cocci and coccobacilli measuring 0.5-0.8μm in diameter. Cells were capsulated. Colonies on solid media were pink colored. The pH range for growth was 3.0-6.0 (optimum pH 4.5). Sugars, gluconate, and some amino acids were good carbon and energy sources for growth. The main components of cellular fatty acids were C15:0 iso and C16:1ω7c. Menaquinone-8 was the major quinone. The G+C content of genomic DNA was 59.5%. Both strains had identical sequences of 16S rRNA genes that were most closely related to that of the type strain of Acidobacterium capsulatum (96% similarity). There were major differences between the isolates and A. capsulatum in cell morphology, carbon nutrition, and fatty acid profiles. Based on these phylogenetic and phenotypic data, we propose the name Acidipila rosea gen. nov., sp. nov. to accommodate the novel isolates. The type strain is AP8T (NBRC 107607T, KCTC 23427T). © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.