The attractant bioactivity test of semi-polar fraction of the datuan stem bark (ficus vasculosa wall.ex miq) against warehouse pest (sitophilus oryzae l.)

Abstract

Bioactive isolation was performed on the stem bark of Datuan (Ficus vasculosa Wall.Ex Miq), and extraction was carried out via the maceration method using acetone as a solvent.Furthermore, an attractant bioactivity test was conducted on acetone extract, A-G fraction, and composition of the isolates.The separation and purification via column chromatography produced a D8.3.5.7 fraction in the form of needle crystal of about 50 mg, at a melting point of 136°C-138.7°C.Thin-layer chromatography (TLC) analysis showed a single spot at an Rf value of 0.57 (n-hexane eluent: ethyl acetate 7:3), 0.36 (DCM eluent), and 0.24 (CHCl3 eluent).The isolated compounds were identified using infrared and UV-Vis spectrophotometry, as well as mass spectrometry.The characterization of the infrared spectrum of the isolated compound showed a strong OH goo band at 3461 cm-1 region and the absorption band at 2936.25 cm-1 exhibited a stretch of CH alkanes.These two bands are supported by the vibration at 1378.47 and 1462.55 cm-1 for CH absorption of methyl and methylene.The absorption band in the 1622 cm-1 region showed a stretch of conjugated C=C double bond, which is supported by absorption at 918.96 and 966.22 cm-1 as C-H alkene.The UV-Vis spectrophotometry showed absorption at λmax 263.97 nm A = 0.483, which was the result of electronic transition π → π∗, and at λ 331.0 nm A = 0.274, which was an electronic result of n → π∗.Meanwhile, identification via mass spectrometry that produces isolate has a molecular weight of 414.1 m/e with the formula C29H50O.Therefore, the bioactivity test results on compound D8.3.5.7 had an attractant activity of 71.67% against warehouse pests (Sitophilus oryzae L.) and an interest index of 0.63.