Extraction of Acemannan Polysaccharides Active Substance from Aloe Vera Flesh with Ethanol Solvent

Abstract

This study was conducted to extract Acemannan Polysaccharides from aloe vera flesh using ethanol solvents and obtain the optimum extraction temperature and deposition time of the extraction process. The process was carried out in batches and in a laboratory scale, with the principle of liquid solid extraction in a stirred glass beaker. The extraction temperatures were 25 , 30 , 35 , 40 , and 45 C and the deposition times were 10, 12, 14, 16, and 18 hours. Some other variables were: 50 cc aloe vera juice volume, 200 cc solvent volume with 96% ethanol concentration, 45 minutes stirring time, and 500 rpm stirring speed. The results obtained were: the largest sediment mass, which was 2.1967 gram, was obtained at extraction temperature of 45 C and deposition time of 18 hours. Based on the results of qualitative analysis, the highest acemannnan content was obtained at 14 hours of deposition with a stirring temperature of 40 C and with 515.18 mg/gram glucose content.