Antibacterial activity of isolated human intestinal microbiota Lactobacillus strains against methicillin resistant and susceptible Staphylococcus aureus

Abstract

In the present study, lactic acid bacteria were screened for anti-methicillin resistant activity. Isolation of lactic acid bacteria from 100 faecal samples of healthy adults was conducted using de Man-Rogosa Sharpe agar (MRS) and three Lactobacilli strains were isolated. The three isolates of human intestinal microbiota were identified on the basis of morphological and biochemical characteristics. The incidence of Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus casei were found to be 43.47, 34.78 and 21.73% respectively. The antibacterial activity of these three isolates was determined against five strains of Staphylococcus aureus, including three methicillin resistant Staphylococcus aureus strains. Maximum inhibitory potential was seen in L. casei. Therapeutic efficacy of the lactobacilli strains was evaluated to control the re-emerging MRSA infection.