Abstract
The recombinant aspartic proteinase-like protein (FeAPL1-His6) was overexpressed in the tobacco BY-2 cell line and the expected pepstatin A-sensitive enzymatic activity was confirmed at pH 3.0. Immunocytochemistry and protein gel blot analysis of the transformed BY-2 cells and their protoplasts showed extracellular localization of rFeAPL1-His6 in the cell wall. Based on the obtained results, potential functions of FeAPL1 are discussed. © 2011 (CC) SCS.
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Milisavljević, M. D., Timotijević, G. S., Nikolić, D. B., Samardžić, J. T., & Maksimović, V. R. (2011). Cell wall localization of the aspartic proteinase from buckwheat (FeAPL1) over-expressed in tobacco BY-2 cells. Journal of the Serbian Chemical Society, 76(9), 1229–1236. https://doi.org/10.2298/JSC110120106M
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