Novel Electrochemical Lactate Biosensors Based on Prussian Blue Nanoparticles †

Abstract

We report on the novel electrochemical lactate biosensors based on Prussian blue nanoparticles. The immobilization of lactate oxidase was performed through drop-casting on the sensor surface of a mixture containing enzyme, (3-aminopropyl)triethoxysilane and isopropyl alcohol. The apparent Michaelis constant and inactivation constant were determined (0.29 ± 0.03 mM and 0.042 ± 0.002 min−1, respectively) and compared with values obtained for biosensors based on Prussian blue films. The developed lactate biosensors are not inferior in characteristics to those previously known, while the manufacturing process is less laborious. Obtained values also indicate that lactate biosensors based on Prussian blue nanoparticles and lactate oxidase have sufficient sensitivity and operational stability for analytical application in medical and biological research.