SNP-based method for the genetic identification of ramin Gonystylus spp. Timber and products: Applied research meeting CITES enforcement needs

Abstract

We describe the development of a genetic assay for the identification of the tropical hardwood ramin Gonystylus spp., a CITES-listed genus subject to illegal international trade. Samples representing different ramin species, confamilial genera and morphologically similar taxa were obtained. DNA extraction from leaf material and wood products was achieved using commercially available kits. Five chloroplast genes were examined for Single Nucleotide Polymorphism (SNP) loci capable of discriminating ramin. A locus within the matK gene was selected and ®a TaqMan assay designed for sample genotyping. The assay was validated against different species to demonstrate its specificity and reproducibility. The final assay provides a robust, cost-effective, transferable method for identifying processed ramin. The research represents a feasibility study, addressing each of the steps required to develop a genetic identification assay for enforcement use; however, it should be noted that further work is required to produce a fully validated forensic identification tool. © Inter-Research 2008.