Blue colored pigment phycocyanin extraction from Spirulina platensis

Abstract

Phycocyanin is a blue pigment and water-soluble biliprotein from the Spirulina platensis. In this study, the water was used as a solvent for the extraction of phycocyanin. Wet biomass of Spirulina, wet biomass held for 48 hours at room temperature, frozen Spirulina for 48 hours, and frozen biomass for fifteen days, and dried Spirulina was used to determine phycocyanin concentration (PC) (mg mL-1). At the same time, extract purity of phycocyanin (OD615/OD280) and yield (mg g-1) were determined. Phycocyanin yield of 17,6497, 17,1370, and 17,0833 mg g-1 were obtained from frozen Spirulina for fifteen days, wet biomass of Spirulina held for 48 hours, and wet biomass of Spirulina, respectively. The purity ratios (OD615/OD280) of 4.60, 0.81, 0.85, 0.98, and 6.55 were determined for frozen for 15 days, frozen Spirulina for 48 hours, wet biomass of Spirulina (waited for 48 hours), dried Spirulina and wet Spirulina, respectively