Molecular diagnostics and phylogenetic analysis of 'Candidatus phytoplasma asteris' (16SrI- Aster yellow group) infecting banana (Musa spp.)

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Abstract

The presence of phytoplasma in diseased banana plants exhibiting acute stunting symptoms was detected by the polymerase chain reaction using a primer set specific for the 16SrRNA gene of phytoplasma. The amplified DNA fragments of 1500 bp were cloned in pGEMT-Easy plasmid cloning vector and sequenced. The BLASTN and phylogenetic analyses revealed the infecting agents to be the closely related members of the 'Candidatus phytoplasma asteris' (16Sr I-Aster yellow) group. © 2009 Academic Journals.

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Singh, B. R., Aminuddin, Al-Khedhairy, A. A., Al-Qurainy, F., & Musarrat, J. (2009). Molecular diagnostics and phylogenetic analysis of “Candidatus phytoplasma asteris” (16SrI- Aster yellow group) infecting banana (Musa spp.). African Journal of Biotechnology, 8(21), 5819–5824. https://doi.org/10.5897/ajb09.1046

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