Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: Genomic complexity and molecular evolution of the gene

Abstract

Full length cDns encoding the glycolyti enzme glyceraldehyde-3-phosphate dehyrogense (GAPDH)fron rat man have been in both genomes based on genmic blot hybridization analysis. Only one functional gene product is know. Result fron genomic library screeings suggest that are 300-400 copies of these sequences in the rat genome and approximately 100 in the human genome, Some of these realted sequences have been shown to be thesepseudogenes indicating that some pseudogenes are transcrined. Rat and human cDNA are 89% homologous in the coding region, and 76% homologous in the frist 100 base paris of the 3' -noncoding region. Comparison of these two cDNA sequences with those of the chicken, Drosophils and yeast genes allows analysis of the evolution of the GAPDH genes in detail. © 1985 IRL Press Limited.