Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application

Abstract

We present a new biosensor for hydrogen peroxide (H 2 O 2 ) detection. The biosensor was based on the immobilization of horseradish peroxidase (HRP) enzyme on layered double hydroxides- (LDH-) modified gold surface. The hydrotalcite LDH (Mg 2 Al) was prepared by coprecipitation in constant pH and in ambient temperature. The immobilization of the peroxidase on layered hybrid materials was realized via electrostatic adsorption autoassembly process. The detection of hydrogen peroxide was successfully observed in PBS buffer with cyclic voltammetry and the chronoamperometry techniques. A limit detection of 9 μM of H 2 O 2 was obtained with a good reproducibility. We investigate the sensitivity of our developed biosensor for H 2 O 2 detection in raw milk.