Boosting of activity enhancement of K+-responsive quadruplex hammerhead ribozyme

Abstract

Two second-generation quadruplex hammerhead ribozymes, whose activity enhances in response to K+via quadruplex formation of embedded r(GGA)3GG, were developed. Different strategies were applied to suppress basal activity when K+ is absent. As a result, the activity enhancement upon the addition of K+ has reached as high as 21-fold.